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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: All- Trans Retinoic Acid Modulates DNA Damage Response and the Expression of the VEGF-A and MKI67 Genes in ARPE-19 Cells Subjected to Oxidative Stress
doi: 10.3390/ijms17060898
Figure Lengend Snippet: Mean intracellular reactive oxygen species (ROS) levels and DNA damage in all- trans retinoic acid (ATRA)-treated and control ARPE-19 cells exposed to tert -butyl hydroperoxide (tBH). Reactive oxygen species (ROS) are expressed as the relative fluorescence of 2′,7′-dichlorofluorescein (DCF) normalized to controls after 30 min incubation with tBH at 37 °C. DNA damage was measured as a percentage of tail DNA in the comets in alkaline versions of the comet assay following exposure to tBH for 15 min at 37 °C. The induction of DNA double strand breaks was assessed by the extent of phosphorylation of H2AX and ATM (ataxia teleangiectasia mutated) (γH2AX and pATM) after γ-ray irradiation (2 Gy/min) of cells. Results are presented as mean ± SEM, n = 12 for ROS level, n = 100 for comet assay, n = 8 for H2AX/ATM phosphorylation, *** p < 0.001.
Article Snippet: The activation of ATM and H2AX was determined using the
Techniques: Control, Fluorescence, Incubation, Single Cell Gel Electrophoresis, Phospho-proteomics, Irradiation
Journal: International Journal of Molecular Sciences
Article Title: All- Trans Retinoic Acid Modulates DNA Damage Response and the Expression of the VEGF-A and MKI67 Genes in ARPE-19 Cells Subjected to Oxidative Stress
doi: 10.3390/ijms17060898
Figure Lengend Snippet: Time course of DNA repair in ATRA-treated and control ARPE-19 cells exposed to 180 μM tert -butyl hydroperoxide (tBH) for 15 min at 37 °C. After the exposure, the oxidant was washed out and the cells were incubated in medium at 37 °C for indicated time points. DNA damage was assessed as percentage of tail DNA in the comet in the alkaline version of the comet assay. Results are presented as mean ± SEM, n = 100, *** p < 0.001.
Article Snippet: The activation of ATM and H2AX was determined using the
Techniques: Control, Incubation, Single Cell Gel Electrophoresis